Figure 10.

CS-SeNPs and Na₂SeO₃ suppressed LPS-induced activation of NF-κB signaling pathway in ECs. After pretreatment with or without 0.5 μM CS-SeNPs or Na₂SeO₃ for 12 h, cells were stimulated with LPS for another 12 h. Western blot analysis was done to determine the protein levels of IκBα, phosphorylated IκBα (P-IκBα), p65, phosphorylated p65 (Ser 536) (P-p65) and β-actin. (a) Representative Western blot bands. (b–e) Quantification analysis of IκBα (b), P-IκBα (c), p65 (d) and P-p65 (e) normalized to β-actin. The results were expressed as mean ± SD (n = 4). ^### p < 0.001, compared with the control cells (Con). * p < 0.05, ** p < 0.01, *** p < 0.001, compared with cells treated with LPS alone.