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. 2021 Oct 28;22(21):11666. doi: 10.3390/ijms222111666

Figure 6.

Figure 6

Bioactivity of CXCL12 released from the Alg microbeads: (A) Visualization of Jurkat cells that migrated in a CXCL12-free cultivation medium and upon stimulation with the CXCL12 released from the microbeads (a confocal microscopy); (B) The calculated amount of CXCL12 released from the microbeads into 100 µL of a PBS/0.1 wt. % BSA solution used for in vitro experiments (an ELISA determination); (C) Migration of Jurkat cells stimulated by CXCL12 released from the microbeads and by a free CXCL12 added into the cultivation medium (positive controls). Values represent the number of migrated cells expressed as % to the control (the means and standard deviation, n = 5). For the microbead composition see Table 1. One-way analysis of variance, the Student–Newman–Keuls method was used for statistical analysis. Indicators above the columns show statistical differences compared to the samples identified with number indicated. The data is expressed as the mean ± SD, * refers to p < 0.001, ** to p < 0.01, $ to p < 0.05.