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. 2021 Oct 29;22(21):11744. doi: 10.3390/ijms222111744

Figure 2.

Figure 2

Level of representative subunits of the complexes in control and hyperthyroid rats. (A). Representative Western blot of OXPHOS subunits (I-ATP5A (CV), II-UQCRC2 (CIII), III-MTCO1 (CIV), IV-SDHB (CII) and V-NDUFB8 (CI)) was detected in isolated heart mitochondria (10 μg of protein/lane) by using the Total OXPHOS Rodent WB Antibody Cocktail. HRM-rat heart mitochondria western blot control, C1-C5-heart mitochondria from the control rats; H1-H4-heart mitochondria from the hyperthyroid rats. (B). Bar graphs represent the levels of appropriate complexes with respect to the Tom 20 level in absolute units. CR-control, HR-hyperthyroidism * p < 0.05, ** p < 0.02, *** p < 0.001 compared with the corresponding control data (n = 15 in each group).