Table 2.
Respiration parameters of heart mitochondria of control and hyperthyroid rats.
| Succ + glu | Glu + mal | |||||
|---|---|---|---|---|---|---|
| CR | HR | CR | HR | |||
| V4(0) | 44 ± 1.1 | 45 ± 1.5 | - | 10.7 ± 0.3 | 10.8 ± 0.3 | - |
| V3 | 211 ± 6.5 | 201 ± 7 | - | 94 ± 2.4 | 94 ± 2.8 | - |
| V4(1) | 46 ± 1 | 53 ± 2 * | 15%↑ | 12.5 ± 0.5 | 16 ± 0.5 ** | 24%↑ |
| VDNP | 210 ± 6.5 | 192 ± 8 | - | 97 ± 4.3 | 93.5 ± 2.6 | - |
| RCR (V3/V4) | 4.5 ± 0.1 | 3.9 ± 0.1 ** | 15%↓ | 7.5 ± 0.2 | 6.2 ± 0.2 *** | 21%↓ |
| t, s | 67 ± 3.3 | 76 ± 3 * | 13%↑ | 50.2 ± 1.3 | 57.1 ± 2.1 * | 14%↑ |
V4(0), V3, V4(1), VDNP-rate of respiration in different metabolic state-nmol O2/min·mg of protein, t-sec. Additions: 5 mM succinate + 5 mM glutamate, 5 mM glutamate + 5 malate, 0.2 mM ADP, 0.05 mM DNP, Mitochondrial protein: 0.25 mg/mL in case of succ + glu or 0.5 mg/mL in case of glu + mal. CR-control, HR-hyperthyroidism. Changes induced by thyroxine: ↑—increase, ↓—decrease, -no change. * p < 0.05, ** p < 0.01, *** p < 0.001, the difference is statistically significant (n = 15–20 in each group).