Table 3.
Respiration parameters of heart mitochondria of control and hyperthyroid rats.
| Palm-carn + mal | TMPD + asc | |||||
|---|---|---|---|---|---|---|
| CR | HR | CR | HR | |||
| V4(0) | 29 ± 1.5 | 31 ± 1 | - | 229 ± 4 | 212 ± 7 | - |
| V3 | 265 ± 5 | 247 ± 5.2 * | 7%↓ | 337 ± 5.5 | 303 ± 9.5 * | 11%↓ |
| V4(1) | 32 ± 0.6 | 41 ± 1.4 *** | 28%↑ | 217 ± 5 | 206 ± 6.5 | - |
| VDNP | 280 ± 7 | 261 ± 3.9 * | 7%↓ | 341 ± 5.5 | 302 ± 8.5 ** | 13%↓ |
| RCR (V3/V4) | 8 ± 0.2 | 6.2 ± 0.2 *** | 29%↓ | 1.6 ± 0.02 | 1.5 ± 0.03 * | 7%↓ |
| t, s | 38.2 ± 1.1 | 48 ± 1.8 *** | 26%↑ | 82 ± 2.8 | 98 ± 4.6 | - |
V4(0), V3, V4(1), VDNP-rate of respiration in different metabolic state-nmol O2/min·mg of protein, t-s. Additions: 0.02 mM L-palmitoylcarnitine + 2.5 mM malate, 0.5 mM TMPD + 2 mM ascorbate, 0.2 mM ADP, 0.05 mM DNP, Mitochondrial protein, 0.5 mg/mL in case of palm-carn + mal or 0.15 mg/mL in case of TMPD + asc. CR-control, HR-hyperthyroidism. Changes induced by thyroxine: ↑—increase, ↓—decrease, -no change. * p < 0.05, ** p < 0.01, *** p < 0.001, the difference is statistically significant (n = 15–20 in each group).