Figure 4.

Poorly aggressive melanoma cells show increased Wnt activity after exposure to normal skin cells. Results from WB and densitometric analysis of WB bands show decreases in the ratio of P-β-catenin to total β-catenin (P-β-cat to β-cat), indicating reduced degradation of β-cat, which would lead to increased potential for Wnt signaling in WM1552C (A) and UACC1273 (B) co-cultured with normal human epidermal keratinocytes (KeraCC) and normal dermal fibroblasts (FibroCC) compared to reference cells (* p < 0.05). Results are representative of at least two independent experiments, each performed in triplicate.