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. 2021 Oct 26;22(21):11570. doi: 10.3390/ijms222111570

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Invasive potential of MCF-7 cells shown by Matrigel invasion assay, glycosylation and EMT characteristics following 24 h CCCM or ICCM transfer. (A) The data presented as a mean of a total number of invaded cells, and the percentage of HPA labelling and EMT markers (vimentin and E-cadherin) positive cells. The error bars represent the SEM of invaded cells and the percentage of HPA and EMT markers of 3 independent experiments (*** p ≤ 0.0001); (B) Representative images for HPA labelling and EMT markers (vimentin and E-cadherin) assays in CCCM or ICCM incubated MCF-7 cells. Scale bar: 100 μm.

Invasive potential of MCF-7 cells shown by Matrigel invasion assay, glycosylation and EMT characteristics following 24 h CCCM or ICCM transfer. (A) The data presented as a mean of a total number of invaded cells, and the percentage of HPA labelling and EMT markers (vimentin and E-cadherin) positive cells. The error bars represent the SEM of invaded cells and the percentage of HPA and EMT markers of 3 independent experiments (*** p ≤ 0.0001); (B) Representative images for HPA labelling and EMT markers (vimentin and E-cadherin) assays in CCCM or ICCM incubated MCF-7 cells. Scale bar: 100 μm.