Table 1.
Forward and reverse primer sequences used for qPCR detection.
| Target Gene | Primer Sequence (5′-3′) | |
|---|---|---|
| Vimentin | F: ATGGCTCGTCACCTTCG R: AGTTTCGTTGATAACCTGTCC |
Primers for EMT related genes |
| E-cadherin | F: ACGCATTGCCACATACA R: CGTTAGCCTCGTTCTCA |
|
| TGFβ-1 | F: TAAAGGGTCTAGGATGCGCG R: GACTTTTCCCCAGACCTCGG |
|
| SLUG | F: AGCAGTTGCACTGTGATGCC R: ACACAGCAGCCAGATTCCTC |
|
| SNAIL | F: AATCGGAAGCCTAACTACAGCG R: GTCCCAGATGAGCATTGGCA |
|
| ZEB1 | F: TCCCTGCCAAGAACAATGATCA R: AGGTGATGGGGATGGTGTACTA |
|
| TWIST | F: ACAGCCGCAGAGACCTAAAC R: GGCCTGTCTCGCTTTCTCTT |
|
| GalNAc-T6 | F: AGAGACAGGGCAGAGGGTAG R: CCTTTGTCATGGCATCCCCT |
Primers for glycosylation related gene |
| has-Let -7a | F: GGGGCTAATACTGCCTGGTAA R: TTCACAATGCGTTATCGGATGT |
Primers for exosomal miRNA |
| has-miR-200b | F: GTTAGAATTAGGGTTTTTGGGGAGG R: ACCTATCAAACTTCTCAATATAAAC |
|
| has-miR-30a | F: GGGATTCTGAAGGTGGGTGG R: AAGAGAGGCAGCTTTCACCC |
|
| has-miR-9f | F: CCAAGCTTATAAGTGAGCGCATTC R: CGGAATTCGTGTTGGAGAACAGCA |
GalNAc-T6: N-acetylgalactosaminyltransferase-6 (also known as ppGalNAc-T6); TGFβ-1: transforming growth factor β1; F: forward; R: reverse.