Figure 5.

Protein identification and Reactome pathway analysis suggest a potential role for endothelial involvement in the aortic calcification process in mice. Reactome pathway analysis was performed from a Zero-order human PPI network using a specific aortic protein database. A hybrid score (negative log10 of enrichment probability multiplied by the enrichment ratio) was assigned for each specific pathway consisting of a subset of input proteins and bridging proteins. (a). Expression of aortic Vdac1 was visualized using Western blotting (b) and total lane protein normalization (Post-transfer) was done using ImageLab (Biorad) software (c) (Control: n = 4, Warfarin: n = 4). Two-tailed Mann Whitney U test was used to assess statistical significance between two groups (c). Significance vs control: p ≤ 0.05: *. Lines and error bars represent mean ± SD.