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. 2021 Oct 22;22(21):11396. doi: 10.3390/ijms222111396

Figure 3.

Figure 3

Intermittent hypoxia induced activation of the ERK, PI3K, PKC and NF-κB signal pathways in the THP-1 cells. The THP-1 cells were treated with normoxia or six cycles of intermittent hypoxia and the culture medium was collected for the enzyme-linked immunosorbent assay. PD98059, LY294002, bisindolylmaleimide I hydrochloride, Bay11-7082 and PX-478, inhibitors specific for the ERK, PI3K, PKC, NF-κB and HIF-1α pathways, respectively, were then used to pretreat the monocytic THP-1 cells one hour before the condition of intermittent hypoxia. The results demonstrated that pretreatment with either 10 μM PD98059, 20 μM LY294002, 2 μM bisindolylmaleimide I hydrochloride and 5 μM Bay11-7082 diminished the IL-8 production induced by intermittent hypoxia. Note: The data were presented as the means and the standard errors from three independent experiments, * p < 0.05 vs. normoxia; † p < 0.05 vs. six IH cycles; ‡ p < 0.05 vs. six IH cycles + PX 10 μM. Abbreviations: BAY = Bay11-7082; BH = bisindolylmaleimide I hydrochloride; IH = intermittent hypoxia; IL = interleukin; LY = LY294002; PD = PD98059; PX = PX-478.