Figure 13.

Flow cytometric analysis of BDCA-2⁺CD123⁺PD-L1⁺ and BDCA-2⁺CD123⁺PD-L2⁺ dendritic cells in the PF of ovarian cancer patients. An acquisition gate was established based on FSC and SSC that included mononuclear cells (P1 population; (A)). Next, the P1 gated MNCs were analyzed for BDCA-2⁺CD123⁺ (P2 population; (B)). The final dot plots of PD-L1 pDCs (region Q2, (C2)) or PD-L2 positive BDCA-2⁺CD123⁺ cells (region Q2-1, (D2)). Fluorescence minus one (FMO) control ((C1,D1), respectively) was used to verify the staining specificity and as a guide for setting the markers to delineate positive populations.