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. 2021 Oct 30;22(21):11802. doi: 10.3390/ijms222111802

Figure 4.

Figure 4

Effects of ω-3 PUFA on brain damage in ischemia-reperfusion (IR) injured Fat-1 mice. (A) Representative immunofluorescence stain of the subgranular zone of the hippocampal DG of the brain section: Immunofluorescent staining was performed using Ki67 in the brain. High power field (HPF), original magnification, 200×. Scale bar = 50 μm. (B) Representative immunofluorescence stain of the subgranular zone of the hippocampal DG of the brain section: Immunofluorescent staining was performed using NeuN in the brain. Original magnification, 400×. Scale bar = 200 μm. (C) Representative Western blot of brain lysis: The brain of uremic Fat-1 mice shows decreased Bax, cleaved caspase3 expression, and increased Bcl2 expression compared to the brain of uremic mice. Additionally, PARP (85 kDa) expression was also reduced in the brain of uremic Fat-1 mice compared to the brain of uremic mice. (D) Representative TUNEL stain of the subgranular zone of the hippocampal DG of the brain section. Original magnification, 200×. Scale bar = 50 μm. * p < 0.001 vs. wt, # p < 0.001 vs. IR. Bar represents mean ± S.D. (wt, wild-type sham; Fat-1, Fat-1 induction sham; IR, IR renal injury in wild-type mice; Fat-1+IR, IR renal injury in Fat-1 induction mice).