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. 2021 Oct 24;22(21):11459. doi: 10.3390/ijms222111459

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Development of stable BEAS-2B cells overexpressing CC16. (A) Immunofluorescence staining images of CC16 were captured in control BEAS-2B and BEAS-2B-CC16. (B) The protein expression of CC16 was analyzed by Western blot. (C) The mRNA levels of CC16 in BEAS-2B and BEAS-2B-CC16 were measured by real-time PCR. (D) The protein levels of CC16 in BEAS-2B and BEAS-2B-CC16 were analyzed by ELISA. Scale bars, 100 µm. Results represent mean ± SD of 3 independent experiments. ** p < 0.01.