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. 2021 Oct 27;22(21):11633. doi: 10.3390/ijms222111633

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Growth-inhibitory effects of MTX on GSCs and differentiated GSCs. GSCs (GS-Y01, GS-Y03) and differentiated GSCs (dGS-Y01, dGS-Y03) treated with MTX at the indicated concentrations for 3 days were subjected to the WST-8 assay. Their viability relative to control was measured. Values in the graphs represent means + standard deviation from triplicate samples of a representative experiment. Similar results were obtained from two independent biological replicates. * p < 0.05 vs. each concentration MTX-treated GSCs by Student’s t-test.