Figure 8.
Evaluation of insulin mRNA and expression of GLUT4 and IRAP molecules in the basolateral amygdala of the mouse brain. (A) Insulin mRNA in arbitrary units (AU). (B) Number of GLUT4/IRAP + cells. (C) Triple immunofluorescent staining: cell nuclei—DAPI (blue), GLUT4 expression (green), IRAP expression (red), signal overlay (Merge). Scale Bar—200 µm. (D) Superposition of signals with triple immunofluorescent staining: cell nuclei DAPI (blue), GLUT4 (green), IRAP (red). Scale Bar—100 microns. WT—control mice group, NLRP3 KO—NLRP3 knockout mice.