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. 2021 Oct 26;22(21):11554. doi: 10.3390/ijms222111554

Figure 3.

Figure 3

Comparison of different methods of labeling blood vessels in various organs with lectins: WGA intravascular delivery during terminal perfusion vs. LEA histological staining on free-floating sections. (A–X) Representative images of the whole-mount dura mater meninx, skeletal muscle, kidney, spinal cord, heart, and liver tissues of the mice intracardially perfused with the WGA lectin (concentration: 50 µg/mL) compared with those histologically stained with the LEA lectin. White squares indicate the point where the high magnification images of the blood vessels used for the quantification were acquired from. Scale bars: 1000 µm for low magnification images, 10 µm for high magnification images. (Y–AD) Quantification of the signal-to-noise ratio values of the two methods in different organs (dura mater: Welch’s t-test, p = 0.3955; skeletal muscle: Mann–Whitney U test, p = 0.2286; Kidney: Welch’s t-test, p = 0.7319; spinal cord: unpaired t-test, p = 0.2778; heart: unpaired t-test, p = 0.5096; liver: unpaired t-test, p = 0.0272); n = 3 to 4 mice for the 50 µg/mL WGA perfusion group and n = 4 mice for the LEA histological staining group. * = p < 0.05.