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. 2021 Oct 26;22(21):11554. doi: 10.3390/ijms222111554

Figure 4.

Figure 4

Comparison of lectin labeling of blood vessels in different types of the endothelium with the commonly used blood vessel markers. (A–D) Representative 20× confocal images of a brain section from a Tie2–eGFP reporter mouse intracardially perfused with the biotinylated lectin and processed with Cy3-conjugated streptavidin and GLUT-1 antibodies. Scale bars: 50 µm. The white dashed square marks the magnified inset in (A′–D′). Scale bars: 10 µm. (E) Quantification of blood vessels labeled (%) by GLUT-1 and lectin staining; n = 4 mice (Wilcoxon test, p = 0.2500). (F–H) Representative 20× confocal images of the brain, kidney, and spleen sections labeled with the CD31 endothelial marker. Scale bars: 50 µm. White dashed squares mark the magnified insets on the right. Scale bars: 10 µm. (I) Quantification of CD31+ blood vessels (%) in the brain, kidney, and spleen tissues that are co-labeled with LEA; n = 3 mice (one-Way ANOVA, p = 0.1894).