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Activity of the nanoformulation and free plasmin with and without α2-antiplasmin with equimolar ratio. Plasmin activity after 30 min treatment with α2-antiplasmin (AP) at 1:1 plasmin:α2-antiplasmin molar ratio. Plasmin nanoformulations (HAP NP) retained significant proteolytic activity in the presence of AP compared to plasmin. Whereas no significant difference was observed between nanoformulation and free plasmin alone. n = 3 + SEM, ** p < 0.01.
