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. 2021 Oct 25;22(21):11502. doi: 10.3390/ijms222111502

Figure 2.

Figure 2

Dual inhibition of Musashi-1 and Musashi-2 impedes cancer stem cell characteristics such as notch pathway gene expression, including pathway elements NOTCH-1 and NOTCH-3 and sphere formation ability. (A): Quantitative polymerase chain reaction (qPCR) results. (B1): Western blot results. (B2): Representative western blot staining. All experiments were repeated at least three times in duplicates. (C1): Representative pictures of sphere formation in PA-1 cells (upper picture: control; lower picture: MSI dual knockdown). (C2): Representative pictures of sphere formation in Caov-3 cells (upper picture: control; lower picture: MSI dual knockdown). (D1): The average size of spheres decreases after MSI dual knockdown in Caov-3 and PA-1 cells. (D2): MSI double knockdown leads to a lower count of cell structures in PA-1 cells per microscopic field of view. p values < 0.05 were deemed significant (* p < 0.05; ** p < 0.01; *** p < 0.001, error bars indicate standard error of the mean (s.e.m.)).