Figure 3.

Aldehyde dehydrogenase activity in Caov-3 cells is downregulated after Musashi-1 and Musashi-2 inhibition. (A): Aldehyde dehydrogenase activity as determined by flow cytometry is reduced after dual inhibition compared to controls. (B): Representative flow cytometric measurements with “negative controls” shown in the presence of the ALDH-inhibitor diethylaminobenzaldehyde (DEAB). (C): ALDH4A1 is downregulated subsequent to dual inhibition, while ALDH1A1 is unchanged in quantitative polymerase chain reaction (qPCR) measurements. All experiments were repeated at least three times in triplets. p values < 0.05 were deemed significant (** p < 0.01; *** p < 0.001; error bars indicate standard error of the mean (s.e.m.)).