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. 2021 Oct 28;22(21):11656. doi: 10.3390/ijms222111656

Figure 4.

Figure 4

Effect of Aldosterone on glioma cell lines and glioma stem cells (A) Quantification of cell numbers in the indicated cell lines in the absence or presence of aldosterone (Aldo) 10−6 M. Data represent the mean ± SEM (n ≥ 3). A colony formation assay is shown as example. (B) qRT-PCR analysis for NR3C2 in T98G cells, T98G cells grown as neurospheres and GSC-11 and GSC-23 patient derived glioma stem cell lines. Data represented as mean fold change of triplicates. (C) Quantification of sphere formation by CSCs cultured in stem cell medium under non-adherent conditions in the absence or presence of ALDO 10−6M Data represent the mean ± SEM (n ≥ 3). * p < 0.05, ** p < 0.01, n.s. = not significant.