Figure 7.

Fly chronic exposure to PQ promotes αSyn proteinase-K-sensitive forms. Heads (n = 100) from αSynWT (a,b) and αSynA53T expressing flies (c,d) at LT50 were digested with proteinase-K (PK) (25 °C, 30 min) at increasing concentrations and analyzed by Western blot with an anti-αSyn (MJFR1) antibody. Cropped images surrounded by dashed lines from the corresponding condition gel with a longer exposition are shown below each gel. Densitometric analyses (b,d) are indicative of 10 independent experiments corresponding to: untreated αSynWT (n = 4), PQ αSynWT (n = 4), untreated αSynA53T (n = 6), and PQ αSynA53T (n = 7). Red stars show the modifications of the PK digestion profile observed in αSynWT (four-pointed stars) or αSynA53T (five-pointed stars) expressing flies. p-values of the group differences were calculated using t-test (* p = 0.0470).