Figure 7.

Water layer blocked physically based anti-melanoma effect: (a) Schematic illustration of the experimental design; (b) water completely inhibited the anti-melanoma effect. Experimental details were briefly illustrated here: Following the protocols in Methods and Materials, the B16F10 cells (1 mL/well) were seeded (0.5 × 10⁴ cells/mL) in a 12-well plate and cultured 24 h before treatment. Helium flow rate was 1.53 lpm. After treatment, 1 mL/well of fresh medium was immediately supplied in the 12-well plate and cells were cultured 3 days before MTT assay. Data were presented as the mean of three independent experiments. Statistical significance was calculated via one-way analysis of variance (ANOVA) followed by Bonferroni post hoc test for multiple comparisons. (c) Representative microscopic imaging (bright field) of cells 1 day after treatment. Scale bar = 50 μm.