Figure 5.

(A) Representative Western blots of costameric and regulatory proteins of soleus and EDL of 10ScSn mice. Samples correspond to soleus (SOL) and EDL muscles from Adult mice (A), vehicle-treated Old mice (O) and curcumin-treated Old mice (O + Cu) (see Supplementary Materials for loading reference). (B) Box plots of normalized values to total loaded protein stained with Red Ponceau (RP). Soleus and EDL data are expressed in arbitrary units relative to the corresponding adult values. Mean and median values are indicated by dotted and solid lines, respectively. n of soleus or EDL samples was A = 4; O = 5 or 6; O + Cu = 6. Comparisons were performed among groups of the same muscle. Indicated p value corresponds to ANOVA test; single or double asterisk indicates the presence of significant difference vs. A, or O, or both, after post-hoc analyses, as detailed in the text.