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. 2021 Oct 29;22(21):11738. doi: 10.3390/ijms222111738

Figure 4.

Figure 4

Gene expression and protein content for key elements of mitochondrial biogenesis in 3T3-L1 adipocytes exposed to CDCA in the absence or presence of TGR5. (A) Representative Western Blot for PGC-1α and TFAM; (B) Gene expression for PGC-1α (ppargc1a), TFAM (tfam), COX I (mt-co1), and mtND5 (mt-nd5). PGC-1a—Peroxisome proliferator-activated receptor gamma, coactivator-1alpha; TFAM—Transcription factor A, mitochondrial; COX I—Cytochrome c oxidase, subunit 1; mtND5—NADH-ubiquinone oxidoreductase, subunit 5. Data are derived from seven independent experiments, and bars represent means ± SEM. * indicates a statistically significant difference vs. Control (p < 0.05); % indicates a statistically significant difference vs. CDCA (p < 0.05); & indicates a statistically significant difference vs. Cas9 (p < 0.05).