Table 2.
Thermocycling conditions for RT-qPCR. Step 3 and 4 were repeated for 40 cycles.
| Step | Temperature (°C; Ramp Rate: 1.6 °C/s) | Time (min) |
|---|---|---|
| 1 | 50 | 2:00 |
| 2 | 95 | 10:00 |
| 3 | 95 | 0:15 |
| 4 | 60 | 1:00 |
| 5 | 95 | 0:15 |
| 6 | 60 | 1:00 |
| 7 | 95 | 0:15 |
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