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. 2021 Oct 28;22(21):11670. doi: 10.3390/ijms222111670

Figure 2.

Figure 2

Doxycycline inhibits the total MMP activity and p38 MAPK activation of ARPE-19 cells. (A) ARPE-19 cells were treated with or without doxycycline (10 μM) and harvested at the indicated times to assay the total MMP activity. The value of cells without drug treatment for 24 h was set as 100%. (B) TGF-β-induced ARPE-19 cells were treated with or without doxycycline (40 μM) for 48 h and assayed for p-p38 MAPK, p38 MAPK, and β-actin via Western blotting. The representative blots (B) and quantitated results (C,D) are shown. In each sample, the values of p-p38 MAPK and p38 MAPK were normalized to that of β-actin and the value of normalized p-p38 MAPK was divided by that of normalized p38 MAPK. The values of cells without TGF-β and doxycycline treatment were set to 1. The data represent means + SEM (error bars) of ≥3 samples per group. Note: *, p < 0.05 and ***, p < 0.001 via Student’s t test.