Figure 5.

Doxycycline and tetracycline treatment decreases p38 MAPK phosphorylation in the retinas and vitreous of mice after PVR induction. (A) The eye sections of naïve mice or mice with PVR induction and treated with saline or doxycycline and tetracycline (D + T) were subjected to immunofluorescence staining with DAPI for the nucleus (blue) and the antibody against p-p38 MAPK (green) 21 days after induction. GCL: ganglion cell layer; INL: inner nuclear layer; ONL: outer nuclear layer. Scale bar, 50 μm. Data are the representative of at least 2 experiments. The retinas harvested from mice as described in (A) were subjected to Western blotting for p38 MAPK, p-p38 MAPK, and β-actin. The representative blots (B) and quantitated results (C,D) are shown. In each sample, the value of p-p38 MAPK was divided by that of p38 MAPK. The values of naïve mice were set as 1. The data represent means + SEM (error bars) of ≥3 samples per group. Note: *, p < 0.05 via Student’s t test.