Table 1.
Antibodies used in this study.
| Name of the Antibody | Clonality | Host | Clone | Catalog Number | Concentration or Dilution Factor | Source |
|---|---|---|---|---|---|---|
| APC mouse anti-human CD11b/Mac1 | Monoclonal | Mouse | ICRF44 | 561015 | 1:5 b | BD Bioscience, Franklin Lakes, NJ, USA |
| APC mouse IgG1κ isotype control | Monoclonal | Mouse | MOPC-21 | 550854 | 1:5 b | BD Bioscience, Franklin Lakes, NJ, USA |
| 6x His tag | Monoclonal | Mouse | GT359 | GTX628914 | 50 μg/mL e | GeneTex, Hsinchu, Taiwan |
| mouse IgG2aκ isotype control | Monoclonal | Mouse | eBM2a | 16-4724-85 | 10 μg/mL c | eBioscience, San Diego, CA, USA |
| mouse IgG3 isotype control | Monoclonal | Mouse | B10 | GTX35027 | 50 μg/mL e | GeneTex, Hsinchu, Taiwan |
| p38α | Polyclonal | Rabbit | N/A a | sc-535 | 1:1000 d | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
| peroxidase-conjugated goat anti-mouse IgG (H+L) | Polyclonal | Goat | N/A a | 115-035-003 | 1:5000 d; 25 µg/mL e |
Jackson ImmunoResearch, West Grove, PA, USA |
| peroxidase-conjugated goat anti-rabbit IgG (H+L) | Polyclonal | Goat | N/A a | 111-035-003 | 1:5000 d | Jackson ImmunoResearch, West Grove, PA, USA |
| phospho-ERK1/2 | Monoclonal | Mouse | E-4 | sc-7383 | 1:3000 d | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
| phospho-p38 MAPK (Thr180/Tyr182) | Monoclonal | Rabbit | D3F9 | 4511 | 1:1000 d | Cell Signaling, Beverly, MA, USA |
| p44/42 MAPK (ERK1/2) | Antiserum | Rabbit | N/A a | M5670 | 1:3000 d | Sigma, St Louis, MO, USA |
| TLR2 | Monoclonal | Mouse | TL2.1 | 16-9922-82 | 10 μg/mL c | eBioscience, San Diego, CA, USA |
| TLR4 | Monoclonal | Mouse | HTA125 | 16-9917-82 | 10 μg/mL c | eBioscience, San Diego, CA, USA |
a N/A: Not available; b Flow cytometry; c Neutralization assay; d Immunoblotting; e ELISA.