Table 2.
Determination of the inhibition type for PAP by comparing the Km and Vmax values between the control systems without inhibitor and the systems containing inhibitors (I) and (II) at a concentration of 0.05 mM. An increase of the Km value with a constant Vmax value in comparison to the basic system indicates the stronger competitive type of inhibition (compound (I)). A constant Km value with a reduced Vmax value in comparison to the basic system indicates the weaker competitive type of inhibition (compound (II)). The results show the mean values for the concentration of 0.05 mM inhibitors (I) and (II) for the 9 substrate concentrations. Each measurement was performed six times (n = 6).
| Control System (without Inhibitor) |
System with Inhibitor (I) | System with Inhibitor (II) | |||||
|---|---|---|---|---|---|---|---|
| Mean | SD | %RSD | Mean | SD | %RSD | ||
| Km [mM] |
1.124 | 2.429 | 0.041 | 1.742 | 2.216 | 0.020 | 1.944 |
| Vmax [mM/min] |
0.600 | 0.597 | 0.007 | 1.242 | 0.598 | 0.079 | 1,261 |
| Type of inhibition | Competitive (I) | Competitive (II) | |||||