KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal anti-PKM2 (D78A4) | Cell Signaling | Cat #4053 |
| Goat polyclonalanti-PFK1 | Santa Cruz | Cat #sc-31712 |
| Mouse monoclonal anti-GAPDH | Santa Cruz | Cat #sc-32233 |
| Rabbit polyclonal to PDHE1a (phospho S293) | Abcam | Cat #ab92696 |
| Rabbit polyclonal to Glutaminase (GLS) | Abcam | Cat #ab93434 |
| Rabbit monoclonal [EPR11370] to GLUD1 | Abcam | Cat #ab168352 |
| Rabbit polyclonal to c-Myc | Santa Cruz | Cat #sc-764 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| YZ9 (1μM) | Cayman Chemical | Cat #15352 |
| KJ-Pyr-9 (20nM) | Cayman Chemical | Cat #19116 |
| CB-839 (100nM) | Cayman Chemical | Cat #22038 |
| O-(Carboxymethyl) hydroxylamine hemihydrochloride (AOA: 0.5mM) | Sigma-Aldrich | Cat #C13408 |
| L-Methionine sulfoximine (MSO: 1mM) | Sigma-Aldrich | Cat #M5379 |
| Etomoxir (5μM) | Sigma-Aldrich | Cat #E1905 |
| UK-5099 (500nM) | Sigma-Aldrich | Cat #PZ0160 |
| Sodium octanoate | Sigma-Aldrich | Cat #C5038-10G |
| U-13C5-L-Glutamine (1 mM) | Cambridge Isotope Laboratories | Cat #CLM-1822-H-0.1 |
| U-13C6-D-Glucose (200μM) | Cambridge Isotope Laboratories | Cat #CLM-1396-PK |
| U-13C3-Pyruvic Acid Sodium Salt (200μM) | Cambridge Isotope Laboratories | Cat #CLM-2440-0.5 |
| U-13C3-L-(+)-Lactic Acid Sodium Salt (5mM) | Cambridge Isotope Laboratories | Cat #CLM-10768-PK |
| U-13C5-alpha-ketoisovaleric acid sodium salt (1 mM) | Cambridge Isotope Laboratories | Cat #CLM-4418-0.25 |
| U-13C2-glycine | Cambridge Isotope Laboratories | Cat #CLM-1017-0.5 |
| U-13C4 L-aspartate acid | Cambridge Isotope Laboratories | Cat#CLM-1801-H-0.25 |
| [1,2]13C2, D-glucose | Cambridge Isotope Laboratories | Cat#CLM-504-0.25 |
| Pregnant Mare Serum Gonadotropin (PMSG) | ProSpec | Cat #hor-272 |
| Chorionic gonadotropin human (hCG) | Sigma-Aldrich | Cat #CG10 |
| α-Ketoisocaproic acid (KIC, 1mM) | Sigma-Aldrich | Cat #68255-1G |
| Sodium 3-methyl-2-oxobutyrate (KIV, 1 mM) | Sigma-Aldrich | Cat #198994-5G |
| Sodium α-ketobutyrate (KB, 1 mM) | Sigma-Aldrich | Cat #K0875-5G |
| α-Ketovaleric acid (KV, 1 mM) | Sigma-Aldrich | Cat #75950-5ML |
| 2-Ketohexanoic acid sodium salt (KH, 1mM) | Sigma-Aldrich | Cat #K6625 |
| β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate (NADH) | Sigma-Aldrich | Cat # N8129-100MG |
| β-Nicotinamide adenine dinucleotide (NAD+) | Sigma-Aldrich | Cat # N8285-15VL |
| Nicotinamide | Sigma-Aldrich | Cat # N0636-100G |
| Nicotinic acid | Sigma-Aldrich | Cat # N0761-100G |
| β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH) | Sigma-Aldrich | Cat # 10107824001 |
| β-Nicotinamide adenine dinucleotide phosphate hydrate (NADP+) | Sigma-Aldrich | Cat # N5755-100MG |
| Critical Commercial Assays | ||
| NADH/NAD glo assay | Promega | Cat #G9071 |
| Deposited Data | ||
| RNA-seq from embryos at 12 developmental time-points cultured using media with and without key nutrients | This study | GSE159484 |
| Experimental Models: Cell Lines | ||
| Mouse: embryo culture | This lab | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: B6C3F1/J | Jackson Laboratory | Stock No: 100010 |
| Software and Algorithms | ||
| Graphpad Prism 7 | GraphPad | http://www.graphpad.com/ |
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| MetaboAnalyst | (Xia and Wishart, 2011) | https://www.metaboanalyst.ca/ |
| Partek software package | Partek Incopr. | https://www.partek.com/ |
| Tracefinder | Thermo. | https://www.thermofisher.com |
| Imaris software package | Imaris | https://imaris.oxinst.com/ |