Table 1.
Cell count, viability, and cell size (µm) of HEK293 cells, as well as AAV5 concentration in viral genomes per mL (vg/mL) 72 h after transfection. N.d. = not detected.
| Sample | Live Cells | Viability | Cell Size | AAV5 Titer | % GFP Positive Cells |
|---|---|---|---|---|---|
| Transfected 1 | 4.00 × 106 | 93.50% | 11.5 | 1.10 × 1010 | 61.9 |
| Transfected 2 | 3.70 × 106 | 94.40% | 12.3 | 4.12 × 109 | 61.7 |
| Transfected 3 | 2.07 × 106 | 91.20% | 10.3 | 1.03 × 1010 | 65.7 |
| Control 1 | 3.24 × 106 | 98.50% | 11.1 | n.d. | n.d. |
| Control 2 | 2.88 × 106 | 96.30% | 9.2 | n.d. | n.d. |
| Control 3 | 5.70 × 106 | 98.30% | 13.2 | n.d. | n.d. |