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In Vivo NL treatment of mice: The experimental study involved 8 weeks (5 days/week) of oral administration of water or nanoliposomes to C57BL/6JRj mice. Lipid extraction from cerebral (cortex and hippocampus) and peripheral (liver) tissues, after freeze-drying, using the Folch method, followed by methylation of fatty acids and analysis of the composition of fatty acids via gas chromatography.
