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(A–D): Effect of chronic 4V OT infusions (16 nmol/day) on adipocyte size in IWAT and EWAT in male HFD-fed CD® IGS rats. Adipocyte size was analyzed using ImageJ. Images were taken from fixed (4% PFA) paraffin-embedded sections (5 μm) containing IWAT (A,B) or EWAT (C,D) in HFD-fed rats treated with 4V OT (16 nmol/day) or 4V vehicle. (A), Veh (IWAT). (B), OT (IWAT). (C), Veh (EWAT). (D), OT (EWAT); (A–D) all visualized at 100× magnification.
