Figure 3.

In vitro, CTSB depletion increased proliferation and modulated cell cycle progression in mouse GC. (A,B) The CCK-8 kit was used to assess the proliferation rate of GCs transfected with siCTSB and NC at 24, 48, and 72 h. The viability of the cells was determined by the concentration of formazan dye, a cell viability indicator. A multimode plate reader with absorbance at 450 nm was used to read the plates, and cell numbers were counted using an automated cell counter. (C–E) Mouse GC was transfected with siCTSB and incubated for 48 h before being saturated in PI, and the FACS and GC cycle were determined. All results are presented as means ± SEM. Significance difference, * p < 0.05, ** p < 0.01, *** p < 0.001; siCTSB, cathepsin B siRNA; GC, granulosa cells; siNC, negative control siRNA; ns, non-significant.