Figure 4.

In vitro effect of CTSB depletion on expression levels (mRNA and protein) of proliferation and cell cycle marker genes in mouse GC. (A) Expression levels of Myc and cyclin D2 genes in GC transfected with siRNA and NC were determined by RT-qPCR against endogenous control β-actin. (B–E) siCTSB promoted the protein expression level of Myc and cyclin D2 in mouse GCs against an endogenous control GAPDH. The data were presented as means ± SEM of three independent experiments. Significant differences, * p < 0.05, ** p < 0.01; siCTSB, Cathepsin B siRNA; GC, granulosa cells; siNC, negative control siRNA.