Table 1.
Primer sequences for quantitative real-time PCR and si-CTSB sequences.
| Gene Name | AC/NO | Primer Sequences | Production Length (bp) | Annealing Temp. (°C) |
|---|---|---|---|---|
| β-actin | NM_007393 | F: GTGACGTTGACATCCGTAAAGA | 287 | 60 |
| R: GTAACAGTCCGCCTAGAAGCAC | ||||
| Caspase 3 | NM_009810 | F: GTCTGACTGGAAAGCCGAAAC | 205 | 59.5 |
| R: GACTGGATGAACCACGACCC | ||||
| Caspase 8 | NM_001080126 | F: TCTCGGAATCGGTAGCAAACC | 173 | 60 |
| R: AGAAGAGCTGTAACCTGTGGC | ||||
| Cyclin D2 | NM_009829.3 | F: TACCTCCCGCAGTGTTCCTA | 158 | 60 |
| R: GCCAAGAAACGGTCCAGGTA | ||||
| MYC | NM_001177352.1 | F: GTTGGAAACCCCGCAGACAG | 264 | 60.5 |
| R: GTAGCGACCGCAACATAGGA | ||||
| CYP11A1 | NM_001346787.1 | F: TACTAACCTAGCCCGCCTCG | 163 | 60.5 |
| R: CTCCTGCGCATAGAGAGAGC | ||||
| FSHR | NM_013523.3 | F: AACACTTGCCAGCCTTTCAC | 184 | 60 |
| R: TGGGTTCCGTTGAATGCACA | ||||
| CTSB | NM_007798.3 | F: CAATGGCCGAGTCAACGTG | 176 | 59 |
| R: TGGTGTATGGTAAGCAGCCT | ||||
| BCL2 | NM_009741.5 | F: GAACTGGGGGAGGATTGTGG | 194 | 60 |
| R: GCATGCTGGGGCCATATAGT | ||||
| LC3-I | NM_025735.3 | F:AGGAGAAGGATGAAGACGGA | 160 | 57 |
| R:CCACTGGGGACTGAAATAGC | ||||
| ATG5 | NM_001358596.1 | F:CAGTGTGATCCCGGCAGA | 199 | 58 |
| R:GAGTAAAGCAAGTTGGAATTCG | ||||
| si-CTSB (204) | F: GCUGUCGGAUGACCUGAUUTT | |||
| R: AAUCAGGUCAUCCGACAGCTT | ||||
| si-CTSB (1096) | F: UCAGAAAUUGUGGCUGGAATT | |||
| R: UUCCAGCCACAAUUUCUGATT |