TABLE 1.
Characteristics of the mammalian SNM1 constructs
| Construct | Vector | Gene | Tag(s)a |
|---|---|---|---|
| hSNM1-HA | pPGK-p(A) | hSNM1 cDNA | HA |
| His-hSNM1-HA | pFastBac1 | hSNM1 cDNA | His10, HA |
| GFP-hSNM1 | pEGFPC2 | hSNM1 cDNA | GFP, His6HA |
| GFP-570hSNM1 | pEGFPC3 | hSNM1 cDNA bp 1–570 | GFP |
| GFP-1515hSNM1 | pEGFPC2 | hSNM1 cDNA bp 1–1515 | GFP |
| GFP-ChSNM1 | pEGFPC3 | hSNM1 cDNA bp 1652–3123 | GFP, His6HA |
| TrcHis-hSNM1 | pTrcHisC | hSNM1 cDNA bp 1930–2285 | His6 |
| mSNM1neo | pBluescript II KS | mSNM1 genomic disruption | NA |
| mSNM1hyg | pBluescript II KS | mSNM1 genomic disruption | NA |
| mSNM1C-GFP | pBluescript II KS | mSNM1-genomic cDNA fusion | GFP |
a
NA, not applicable.