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. 2021 Nov 4;22(21):11961. doi: 10.3390/ijms222111961

Figure 1.

Figure 1

Single-cell migration of breast carcinoma cells on plastic (PL), fibronectin (FN), or laminin (LN) surfaces as revealed by video time-lapse analysis. Histogram shows the velocity of breast carcinoma cells that were analysed for 24 h (given in µm per h + SD). Tracing of the migratory paths was accomplished with the software “Image J” and “CellTracker”. Per cell line and substratum, at least 20 cells were analysed. Selected micrographs show breast carcinoma cells that had been cultivated for 24 h on a plastic (PL), a fibronectin (FN), or a laminin (LN) substratum. Micrographs of identical sections at the beginning (T0) and after 24 h (T24) of the culture period are shown (bar, 50 μm). Notice that in some combinations, such as MCF-7 or SK-BR-3 cells cultivated on PL, almost identical positions of immobile but proliferating cells are present, whereas considerable but variable cell movements occur in other combinations, such as MCF-7 or MDA-MB-231 cells cultivated on LN (also reflected by the large SD values in the histogram).