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. 2021 Nov 4;22(21):11961. doi: 10.3390/ijms222111961

Figure 3.

Figure 3

Single-cell migration of breast carcinoma cells on plastic (PL), fibronectin (FN), or laminin (LN) surfaces in the absence or presence of 50 nM of SSP, as revealed by video time-lapse analysis. (A) Histogram shows the velocity of breast carcinoma cells that were analysed for 24 h (given in μm per h + SD). With the exception of MCF-7 cells on LN and MDA-MB-231 cells on PL, the differences between untreated and SSP-treated cells are statistically significant, as determined by Student’s t-test. ☠: MDA-MB-231 cells on FN did not survive SSP treatment. (B) Selected micrographs of breast carcinoma cells that had been cultivated for 24 h in the presence of 50 nM of SSP on a PL, FN, or LN substratum. Micrographs of identical sections at the onset of the experiment (T0) and 24 h later (T24) are shown. Notice the changed positions of cells of MCF-7 cells cultivated on PL or FN. In each case, the position of two cells is indicated by small or large arrows (bar, 40 μm). Notice the presence of immobile flattened MDA-MB-231 and SK-BR-3 cells cultivated on LN.