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. 1999 Dec;37(12):3917–3924. doi: 10.1128/jcm.37.12.3917-3924.1999

FIG. 2.

FIG. 2

Sensitivity of the single PCR method in detecting virus-specific DNA extracted from 10-fold dilutions (10−1 to 10−6; lanes 1 to 6, respectively) of purified viral preparation of PCV-1 (preadjusted to 106 viral particles per ml) (A and C) or from PK-15 cells infected with PCV-1 (total of 106 cells) (B). The primer pairs used were chosen in order to permit amplification of ORF1 fragments of either 375 bp (primers ORF1.PCV1.S2 and ORF1.PCV1.AS1) or 865 bp (primers ORF1.PCV1.S1 and ORF1.PCV1.AS5) in length. Lane l, 1-kb DNA ladder.