FIG. 3.

PCR products resulting from enzymatic amplification of PCV genomic DNA extracted from infected porcine lung tissue. Clinical samples with histopathological lesions suggestive of a PCV infection (presence of basophilic inclusion bodies) (Table 4) were treated as described in the Materials and Methods section, and then extracted DNA was tested by the single PCR method with primers ORF1.PCV1.S2 and ORF1.PCV1.AS2, which yielded DNA amplicons of 465 bp in length. Lanes 1 to 22, samples from 22 pigs, respectively; lane L, 1-kb DNA ladder; +, DNA from a purified PCV-1 strain.