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. 1999 Dec;37(12):3917–3924. doi: 10.1128/jcm.37.12.3917-3924.1999

FIG. 4.

FIG. 4

Strain specificity of the oligonucleotide primers used in both mPCR methods. As expected from the genomic sequences of PCV-1 and PCV-2, primer pair ORF1.PCV1.S3 and ORF1.PCV1.AS2 and primer pair ORF2.PCV1.S and ORF2.PCV1.AS which were used in the first mPCR method (A) yielded DNA amplicons of 488 bp specific for the ORF2 genes of both genotypes, whereas a 375-bp fragment was amplified for the ORF1 gene of the PCV-1 strain only. In the second mPCR-2 method (B), primer pair ORF1.PCV1.S2 and ORF1.PCV1.AS6 and primer pair ORF2.PCV1.S1 and ORF2.PCV1.AS1 yielded DNA amplicons of 646 bp specific for the ORF1 genes of both genotypes, whereas a 407-bp fragment was amplified for the ORF2 gene of the PCV-1 strain only. Lane L, 1-kb DNA ladder; lanes 1 and 2, genotypes 1 and 2 of PCV, respectively.