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. 1999 Dec;37(12):3917–3924. doi: 10.1128/jcm.37.12.3917-3924.1999

FIG. 6.

FIG. 6

Differentiation of PCV-1 and PCV-2 strains by single PCR with universal and type 2-specific primer pairs. Primer pair ORF1.PCV1.S2 and ORF1.PCV1.AS6 (lanes 1 to 4) yielded a DNA amplicon of 646 bp specific for the ORF1 genes of both genotypes, whereas primer pair ORF2.PCV2.S4 and ORF2.PCV2.AS4 (lanes 5 to 8) yielded a DNA amplicon of 493 bp specific for the ORF2 gene of PCV-2 only. Lanes 1 and 5, DNA extracted from PK-15 cells persistently infected with the PCV-1 strain; lanes 2 and 6, clinical specimen positive for PCV-1 by the mPCR; lanes 3 and 7, Quebec PCV-2 strain IAF-614; lanes 4 and 8, Quebec PCV-2 strain IAF-4370; lanes L, 1-kb DNA ladder; lane +, DNA extracted from purified PCV-1.