TABLE 2.

Sequences of oligonucleotides used for single PCR and their localization on the virus genome^a

Primer	Sense	Sequence (5′–3′)	Genomic position	Template	% Homology between PCV-1 and PCV-2
ORF1.PCV1.S1	+	GCCAAGCAAGAAAAGC	47–63	ORF1	93
ORF1.PCV1.S2	+	GAGGTGGGTGTTCACCCT	82–97	ORF1	94
ORF1.PCV1.AS1	−	CGTTACAGGGAACTGCTC	445–462	ORF1	89
ORF1.PCV1.AS2	−	GTGTACAGCTGTCTTCCAATC	527–547	ORF1	86
ORF1.PCV1.AS5	−	GTGGATTGTTCTCCAGCAGTC	892–912	ORF1	86
ORF1.PCV1.AS6	−	CACACAGTCTCAGTAGATCATCC	706–728	ORF1	100
ORF2.PCV2.S4	+	CACGGATATTGTAGTCCTGGT	1093–1114	ORF2	36
ORF2.PCV2.AS4	+	CCGCACCTTCGGATATACTGTC	1565–1586	ORF2	31

^aNucleotide sequences and localization on the PCV-1 or PCV-2 genome were given in reference to the sequences of the prototype ATCC CCL-33 strain of PCV-1 (GenBank accession no. 49186) (23) and the sequences of the PMWS-associated PCV-2 strain isolated in Manitoba, Canada (GenBank accession no. AF027217) (14).