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. 1999 Dec;37(12):3917–3924. doi: 10.1128/jcm.37.12.3917-3924.1999

TABLE 3.

Description and genomic positions of four sets of primers designed for mPCR according to PCV-1 sequencea

mPCR Primer Sequence (5′–3′) Genomic position Template Amplicon (bp) % Homology between PCV-1 and PCV-2 Specificity for strain:
PCV-1 PCV-2
1 ORF1.PCV1.S3 CGGAGAGGAAGGTTTGGAAG 172–191 ORF1 375 60 +
ORF1.PCV1.AS2 GTGTACAGCTGTCTTCCAATC 527–547 ORF1 86
ORF2.PCV1.S ATGACGTGGCCAAGGAGGCGTTAC 1723–1700 ORF2 488 92 + +
ORF2.PCV.1AS GTGAAGTACCTGGAGTGGTAGG 896–916 ORF2 91
2 ORF1.PCV1.S2 GAGGTGGGTGTTCACCCT 82–97 ORF1 646 94 + +
ORF1.PCV1.AS6 GGATGATCTACTGAGACTGTGTG 706–728 ORF1 100
ORF2.PCV1.S1 GGGTTACAAAGTTGGCATCCAAG 1327–1350 ORF2 407 70 +
ORF2.PCV1.AS1 CACTTTTATAGGATGACGTGG 1714–1734 ORF2 52
a

Nucleotide sequences and their localization on the PCV-1 genome were given in reference to those of the sequence of the prototype ATCC CCL-33 strain of PCV-1 (GenBank accession no. 49186) (23).