TABLE 3.

Description and genomic positions of four sets of primers designed for mPCR according to PCV-1 sequence^a

mPCR	Primer	Sequence (5′–3′)	Genomic position	Template	Amplicon (bp)	% Homology between PCV-1 and PCV-2	Specificity for strain:
							PCV-1	PCV-2
1	ORF1.PCV1.S3	CGGAGAGGAAGGTTTGGAAG	172–191	ORF1	375	60	+	−
	ORF1.PCV1.AS2	GTGTACAGCTGTCTTCCAATC	527–547	ORF1		86
	ORF2.PCV1.S	ATGACGTGGCCAAGGAGGCGTTAC	1723–1700	ORF2	488	92	+	+
	ORF2.PCV.1AS	GTGAAGTACCTGGAGTGGTAGG	896–916	ORF2		91
2	ORF1.PCV1.S2	GAGGTGGGTGTTCACCCT	82–97	ORF1	646	94	+	+
	ORF1.PCV1.AS6	GGATGATCTACTGAGACTGTGTG	706–728	ORF1		100
	ORF2.PCV1.S1	GGGTTACAAAGTTGGCATCCAAG	1327–1350	ORF2	407	70	+	−
	ORF2.PCV1.AS1	CACTTTTATAGGATGACGTGG	1714–1734	ORF2		52

^aNucleotide sequences and their localization on the PCV-1 genome were given in reference to those of the sequence of the prototype ATCC CCL-33 strain of PCV-1 (GenBank accession no. 49186) (23).