Table 1.

Novel variants (false positives): Summary characteristics in the mutserve variant calling method (VCM).

Variable	PCP		TSS
	Primary Novel Variants	Mixture Novel Variants	Primary Novel Variants	Mixture Novel Variants
Normalized coverage	↓	↓↓	↓↓↓	↓↓
Coverage ratio	-	↑↑↑	↑↑↑	↑
Number of NUMTs	↑↑↑	↓	-	↓↓
LCR prevalence	↓↓↓	↓↓↓	↑↑↑	-
Distance to amplicon edge	↓↓↓	↓↓	↓	-

Using found variants (true positives) as a reference: “-“ denotes nonsignificant changes; “↑” or “↓” denote significant changes in the 25–50% range; “↑↑” or “↓↓” denote significant changes in the 51–75% range; and “↑↑↑” or “↓↓↓” denote significant changes > 75% (more details in Tables S17–S21). Abbreviations: PCP—PrecisionCallerPipeline; TSS—Ion Torrent Suite™ Software; NUMTs—nuclear insertions of mitochondrial DNA; LCR—low-complexity region.