Figure 1.

Workflow for the multidisciplinary study (toolbox) for two kinds of plants source materials—C. majus extracts and latex. The research scheme enabled fractionation with the use of affinity chromatography, monitoring of all fractions with the use of proteomic, zymography, as well as LC-ESI-MS/MS techniques for the identification of proteins and non-protein substances. Molecular interactions were confirmed with the use of bioinformatic tools of molecular modeling and docking, and their biological activities were finally analyzed using cell lines.