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. 2021 Oct 31;22(21):11838. doi: 10.3390/ijms222111838

Figure 2.

Figure 2

Fractionation of C. majus whole plant extract on a heparin column. Top: Absorbance at 280 nm—blue line—indicates protein content in fractions. Red lines and numbers represent fraction numbers. Middle: In-gel DNase assay zymography of protein fractions with high nucleolytic activity. Band 1A of ca. MW 33–35 kDa was cut, and MLP presence was confirmed by MS/MS. Bottom: Protein profiles of each fraction in SDS-PAGE stained with silver. Band 1B of ca. MW 36 kDa was cut from the gel, and MLP presence was confirmed by MS/MS.