Table 1.
Compound-induced thermal stabilization or destabilization of the investigated oligonucleotides, measured by circular dichroism melting experiments. ΔT1/2 values are the differences in the apparent melting temperatures of DNA in the presence and absence of compounds.
| Compound | ΔT1/2 (°C) 1 | ||||||
|---|---|---|---|---|---|---|---|
| Hairpin | Bcl-2 G4 | c-Myc G4 | Tel23 G4 | Bcl-2 iM | c-Myc iM | hTeloC iM | |
| 15a | 0 | 5.0 | 7.0 | 1.1 | −7.0 | −18.4 | −12 |
| 16a | −0.2 | 4.5 | 5.0 | 1.5 | −11.0 | −27.5 | −25.0 |
| 17a | −1.0 | 4.0 | 5.0 | 0 | −10.5 | −27.0 | −24.5 |
| 18a | 0.2 | 4.5 | 7.5 | 3.5 | −13.5 | −24.5 | −21.0 |
| 19a | 1.7 | 0.5 | 2.0 | 1.0 | −4.0 | −5.5 | −4.0 |
| 20a | 3.9 | 0.5 | 1.0 | 0 | −8.0 | −10.4 | −18.0 |
| 21a | 2.9 | 0 | 0 | 0 | 0 | −0.8 | 2.5 |
| 22a | −0.2 | 2.7 | 4.5 | 0.5 | −11.0 | −19.0 | −25.5 |
| 23a | 2.9 | 0 | 0.5 | 0.5 | −6.0 | −17.0 | −12.5 |
| 24a | −0.7 | 1.5 | 2.0 | −0.5 | −2.0 | −2.8 | 1.5 |
| 15b | −0.1 | 5.1 | 7.5 | 1.6 | −6.0 | −20 | −26.5 |
| 16b | −0.1 | 4.5 | 8.0 | 1.6 | −13.0 | −19.4 | −24.5 |
| 17b | −0.1 | 4.0 | 5.5 | 1.0 | −10 | −17.4 | −23.5 |
| 18b | −0.1 | 5.5 | 8.5 | 1.5 | −6.0 | −11.4 | −15.5 |
| 19b | 0 | 3.0 | 2.0 | 1.2 | −5.0 | −4.0 | −7.0 |
| 20b | 2.2 | 5.9 | 4.0 | 0.1 | −8.0 | −6.0 | −9.0 |
| 21b | 1.9 | 1.0 | 1.0 | 2.0 | 0 | −0.4 | 2.0 |
| 15c | −0.1 | 6.1 | 10.6 | 0 | −11.0 | −24 | −29 |
| 16c | −0.1 | 5.6 | 9.5 | 0.5 | −10.0 | −17.4 | −24.1 |
| 17c | −0.1 | 5.6 | 6.5 | 2.0 | −12.0 | −21.1 | −27.4 |
| 18c | 1.1 | 7.6 | 10.5 | 1.6 | −10.5 | −20.0 | −25.5 |
| 19c | −1.1 | 5.1 | 4.2 | −1.0 | −3.0 | −3.0 | −10.0 |
| 20c | 3.0 | 7.1 | 6.8 | 0 | −9.0 | −2.9 | −9.0 |
| 21c | 1.9 | 0 | 0 | 2.0 | 0 | −0.9 | 1.5 |
| 15d | 0 | 8.8 | 9.8 | −1.0 | −5.0 | −4.9 | −7.0 |
| 16d | −0.3 | 8.0 | 8.1 | 2.0 | −5.0 | −4.9 | −7.0 |
| 17d | 1.0 | 9.0 | 6.9 | 2.0 | −8.0 | −7.0 | −8.9 |
| 18d | 0 | 10.1 | 9.8 | −1.0 | −8.0 | −8.0 | −9.0 |
| 19d | 0.9 | 4.2 | 2.8 | −2.0 | −5.0 | −5.0 | −7.0 |
| 20d | 2.9 | 8.0 | 8.2 | 0 | −8.0 | −5.0 | −9.0 |
| 21d | 1.9 | 2.0 | 0.8 | −0.8 | −1.0 | 0 | −1.0 |
1 The error on ΔT1/2 values is ±1.0 °C. All experiments were performed in duplicate, and the values reported are the average of two measurements.